Journal: BioMedical Engineering OnLine
Article Title: Endothelial cells influence the osteogenic potential of bone marrow stromal cells
doi: 10.1186/1475-925X-8-34
Figure Lengend Snippet: mRNA Expression after 5 days by real time PCR . There were four groups: Group 1: MSC co-cultured with EC (Co); Group 2: MSC induced by osteogenic stimulatory medium (OM); Group 3: MSC in mono-culture without osteogenic stimulatory supplements (MSC) and Group 4: EC only (EC). Results were reported as fold changes ± fold-difference (2 -ΔΔCt ± 2 -(ΔΔCt ± SD) ). The y-axis showed mRNA level in logarithmic scale for ALP, RunX2 and Col I. A significant upregulation of ALP expression was found in MSC/EC co-cultures compared with MSC alone. All the data were normalized with endogenous control GAPDH. ** P < 0.01 * P < 0.05.
Article Snippet: In order to confirm the Superarray results, real-time PCR was performed independently and 5 day osteogenic stimulatory medium (OM)-induced MSC were used as a positive control (Fig. ).
Techniques: Expressing, Real-time Polymerase Chain Reaction, Cell Culture, Control